サイズ選択用SPRIselect

以下を定める弊社の特許製品であるSPRI常磁性ビーズに基づく化学製品を用い、ライブラリー調製用に希望するサイズのDNA断片を精製します:

  • 特定のアプリケーションに適合する調整可能な断片サイズの選択
  • 測定と試薬ロットの間で予測可能かつ一貫したサイズ選択
  • 手動測定および自動化測定との適合性

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SPRIselectゲノム製品

レフトサイドセレクション プロトコール

Genomics SPRIselect Left Side Size Selection Workflow

ライトサイドセレクション プロトコール

Genomics SPRIselect Right Side Size Selection Workflow

製品特徴

次世代シークエンサーサンプルのサイズセレクションをより高品質に、よりハイスループットに

150bp~800bpの任意の範囲でサイズセレクションが可能

厳しい品質管理により、高い再現性を実現

SPRIselectとマグネットツールのみで、ゲルや特別な装置は一切不要

製品仕様

Application Uses Purification & Clean-up, Nucleic Acid Sample Prep, DNA Size Selection, RNA Size Selection, PCR Clean-up, NGS Clean-up, PCR clean-up
Format Liquid
Starting Sample Material Nucleic Acid
Automated Available Yes
Item Specifications Referenced B23318

Citations

Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-51

  • Size Selection of Libraries

Kubo, M., Nishiyama, T., Tamada, Y., Sano, R., Ishikawa, M., Murata, T., . . . Hasebe, M. (2019). Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation. Nucleic Acids Research, 47(9), 4539–4553. doi:10.1093/nar/gkz1811

  • Used on cDNA Libraries

Behera, V., Stonestrom, A. J., Hamagami, N., Hsiung, C. C., Keller, C. A., Giardine, B., . . . Blobel, G. A. (2019, April 9). Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell Reports, 27, 400–415. doi:10.1016/j.celrep.2019.03.0571

  • Used on ChIP-seq Libraries

SPRIselect vs. AMPure XP Feature Comparison

SPRIselect AMPure XP
Stable at room temperature Stable at 4oC
QC'd for size selection QC'd for recovery
Suggested for use in library construction kits Suggested for use in library construction kits

SPRIselect and AMPure XP

  • Can be substituted for each other in clean-up applications
  • Use the same sample to bead ratios
  • Are suggested for use by the most well-known library construction kit manufactures:
    • Illumnia®, Integrated DNA Technologies, Inc (IDT), Swift Biosciences, etc.
  • Both have identical work flows 

SPRIselect Benefits

  • Stability at room temperature allows:
    • Start clean-up steps without calibrating to room temperature
    • Frees up 4⁰C storage space
  • Quality control for size selection allows:
    • Trust that lot to your size selection steps are accurate
    • Accurate and consistent size selection from lot to lot

1. Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

資料

Title Description
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
{9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}
{9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}
{9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}
{B14306BD-BB46-4B27-BF3E-B11862085BC7}


Technical Documents