SPRIselectによるサイズセレクション

当社独自のSPRI磁性ビーズを用いることにより、ライブラリ調製に望ましいサイズのDNA断片を精製できます。

  • 用途に合わせてDNA断片のサイズを調整できます
  • 複数回の実験や試薬ロットにかかわらず、安定したサイズセレクションが可能で
  • 手動操作および自動化処理に適合

試験成績証明書のダウンロード:ページ上部にある検索窓でロット番号を検索の上、ダウンロードしてください。

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SPRIselect

レフトサイドセレクション プロトコール

Genomics SPRIselect Left Side Size Selection Workflow

ライトサイドセレクション プロトコール

Genomics SPRIselect Right Side Size Selection Workflow

製品特徴

次世代シークエンサーサンプルのサイズセレクションをより高品質に、よりハイスループットに

150bp~800bpの任意の範囲でサイズセレクションが可能

厳しい品質管理により、高い再現性を実現

SPRIselectとマグネットツールのみで、ゲルや特別な装置は一切不要

製品仕様

Application Uses Purification & Clean-up, Nucleic Acid Sample Prep, DNA Size Selection, RNA Size Selection, PCR Clean-up, NGS Clean-up, PCR clean-up
Format Liquid
Starting Sample Material Nucleic Acid
Automated Available Yes
Item Specifications Referenced B23318

Citations

Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-51

  • Size Selection of Libraries

Kubo, M., Nishiyama, T., Tamada, Y., Sano, R., Ishikawa, M., Murata, T., . . . Hasebe, M. (2019). Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation. Nucleic Acids Research, 47(9), 4539–4553. doi:10.1093/nar/gkz1811

  • Used on cDNA Libraries

Behera, V., Stonestrom, A. J., Hamagami, N., Hsiung, C. C., Keller, C. A., Giardine, B., . . . Blobel, G. A. (2019, April 9). Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell Reports, 27, 400–415. doi:10.1016/j.celrep.2019.03.0571

  • Used on ChIP-seq Libraries

SPRIselect vs. AMPure XP Feature Comparison

SPRIselect AMPure XP
Stable at room temperature Stable at 4oC
QC'd for size selection QC'd for recovery
Suggested for use in library construction kits Suggested for use in library construction kits

SPRIselect and AMPure XP

  • Can be substituted for each other in clean-up applications
  • Use the same sample to bead ratios
  • Are suggested for use by the most well-known library construction kit manufactures:
    • Illumnia®, Integrated DNA Technologies, Inc (IDT), Swift Biosciences, etc.
  • Both have identical work flows 

SPRIselect Benefits

  • Stability at room temperature allows:
    • Start clean-up steps without calibrating to room temperature
    • Frees up 4⁰C storage space
  • Quality control for size selection allows:
    • Trust that lot to your size selection steps are accurate
    • Accurate and consistent size selection from lot to lot

1. Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

資料

Title Description
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{B14306BD-BB46-4B27-BF3E-B11862085BC7}
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{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
{9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}


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