サイズ選択 

Agencourt SPRIselectは、次世代シーケンシング向けの断片ライブラリを用意する上で核酸のサイズ選定を高速化・単純化できるSPRIベースの化学製品です。このプロセスではサイズ選定を行い、断片を平均サイズを中心として一様に分布させる必要があります。SPRIselectを使用することで、使用する解析法およびプラットフォームに合わせてサイズ分布を調製できます。本プロセスはワークフローの処理能力に合わせて拡大/縮小できます。SPRIselectはマニュアルまたはBiomekリキッドハンドラーで自動で使用可能であり、ほぼすべての解析法に適したサイズ選定を高速に安定して行えます。

Choose a Size Selection Model

SPRIselect Left Workflow

Genomics SPRIselect Left Side Size Selection Workflow

SPRIselect Right Workflow

Genomics SPRIselect Right Side Size Selection Workflow

製品仕様

Application Uses Purification & Clean-up, Nucleic acid sample prep, DNA Size Selection, RNA Size Selection, PCR Clean-up, NGS Clean-up, PCR clean-up
Format Liquid
Starting Sample Material Nucleic Acid
Automated Available Yes
Item Specifications Referenced B23318

Citations

Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-5

  • Size Selection of Libraries

Kubo, M., Nishiyama, T., Tamada, Y., Sano, R., Ishikawa, M., Murata, T., . . . Hasebe, M. (2019). Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation. Nucleic Acids Research, 47(9), 4539–4553. doi:10.1093/nar/gkz181

  • Used on cDNA Libraries

Behera, V., Stonestrom, A. J., Hamagami, N., Hsiung, C. C., Keller, C. A., Giardine, B., . . . Blobel, G. A. (2019, April 9). Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell Reports, 27, 400–415. doi:10.1016/j.celrep.2019.03.057

  • Used on ChIP-seq Libraries

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Documentation and Related Materials



Technical Documents