垂直ロータ

垂直ロータは、どのロータタイプより最短の沈降経路長と遠心時間、および最高の分解能を特徴としています。垂直ロータでは、遠心中、チューブは垂直です。チューブの横幅が沈降経路長になり短いため、短時間での分離が可能になります。完全に垂直なチューブの中で遠心力はチューブの外壁全体に沿ってペレットを広げるため、ペレッティングに使用するのに適したロータではありません。

垂直ロータの使用例は以下の通りです：

等密度遠心－塩化セシウム中のDNAのバンディングに特化
ウイルス粒子などの密度勾配遠心

密度勾配遠心：

チューブの中で等密度の位置に粒子が集まってバンドを形成します。
「軟質」クッション界面でのペレット化サンプルの分離に理想的

垂直ロータの使用目的と仕組みの詳細はこちら

垂直ロータ VTi 50.1

使用可能機種：Optima XPN / XE
最高回転数 – 50,000 rpm
最大遠心力 – 251,000 xg
kファクタ値 – 34
チューブの本数 x 容量／寸法：
- 12 x 39 mL
- 25 x 89 mm
ロータ容量 – 468 mL

垂直ロータ VTi 65.1

使用可能機種：Optima XPN / XE
最高回転数 – 65,000
最大遠心力 – 42,000 x g
kファクタ値 – 13
チューブの本数 x 容量／寸法：
- 8 x 13.5 mL
- 16 x 76 mm
ロータ容量 – 108 mL

垂直ロータ VTi 65.2

使用可能機種：Optima XPN / XE
最高回転数 – 65,000 rpm
最大遠心力 – 416,000 xg
kファクタ値 – 10
チューブの本数 x 容量／寸法：
- 16 x 5.1 mL
- 13 x 51 mm
ロータ容量 – 81.6 mL

垂直ロータ VTi 90

使用可能機種：Optima XPN / XE
最高回転数 – 90,000 rpm
最大遠心力 – 645,000 x g
kファクタ値 – 6
チューブの本数／容量／寸法／形状
- 8 x 5.1 mL
- 13 x 51 mm
ロータ容量 – 40.8 mL

FAQ on Vertical Angle Rotors

What applications are vertical rotors commonly used for?

Vertical rotors are commonly used for isopycnic centrifugation, density gradient separations, plasmid DNA purification, viral particle isolation, lipoprotein analysis and other ultracentrifugation workflows that require rapid equilibrium separations.

What is a vertical rotor?

A vertical rotor is an ultracentrifuge rotor that maintains sample tubes parallel to the axis of rotation during centrifugation. This geometry creates the shortest sedimentation path of any rotor design and helps accelerate density gradient separations.

Why are vertical rotors used for isopycnic centrifugation?

Vertical rotors provide a very short path length through the density gradient. This allows particles to migrate quickly to their equilibrium density, helping reduce run times while maintaining high separation resolution.

What is the k-factor of a centrifuge rotor?

The k-factor is a measure of a rotor's sedimentation efficiency. Lower k-factor values generally indicate faster particle sedimentation and shorter run times. When comparing ultracentrifuge rotors, the k-factor can help determine which rotor may provide the most efficient separation for a specific application.

Are vertical rotors suitable for pelleting applications?

Vertical rotors are generally not the preferred choice for pelleting. Because the tubes remain vertical during centrifugation, pellets may spread along the outer wall of the tube rather than forming a compact pellet at the bottom. Fixed-angle or swinging-bucket rotors are typically better suited for pelleting workflows.

What is the difference between a vertical rotor and a fixed-angle rotor?

Vertical rotors are primarily designed for density gradient and isopycnic separations, where particles move through a gradient to reach their equilibrium density. Fixed-angle rotors are typically optimized for pelleting applications, where particles sediment toward the tube wall and form a pellet. The choice depends on whether the goal is particle pelleting or high-resolution gradient separation.

When should I use a near vertical rotor instead of a vertical rotor?

Use a near vertical rotor instead of a vertical rotor if particles in your solution will pellet out of solution and risk contaminating your gradient bands. In a near vertical rotor, those containments will move to the bottom of the tube instead of remain on the side wall, as they do in a vertical rotor.