GenFind V3

血液からのDNA抽出

GenFind V3はSPRI磁性ビーズを用いた、血液（全血、血漿、血清）からのDNA抽出に対応する試薬キットです。血液サンプルからのRNA抽出はRNAdvance Blood、血漿・血清からのcfDNA抽出はApostle MiniMax™ cfDNAがそれぞれ対応製品となります。

  • 血液（全血、血漿、血清）400μLから、高品質・高収量なDNA抽出。
  • 抗凝固剤や凍結保存の影響を受けづらく、PCRやNGSなどのアプリケーションにも最適。
  • 追加プロトコルで、ろ紙血、だ液、洗口液、細菌などからのDNA抽出に対応。

安全性データシート（SDS）は、こちらからダウンロードしてください。

核酸抽出・精製試薬キットのカタログはこちら。

本製品の使用にはマグネットアクセサリが必要です。

GenFind V3 Genomic DNA Extraction Kit

製品モデル

GenFind V3 gDNA Extraction Workflow

GenFind V3 high molecular weight genomic DNA extraction workflow
  1. Lyse the sample
  2. Bind DNA to SPRI beads
  3. Separate beads from contaminants
  4. Wash beads to remove contaminants
  5. Elute DNA from beads
  6. Transfer to new plate

Data and Performance

High recovery of high molecular weight genomic DNA with GenFind V3 kit

High Molecular Weight Genomic DNA Extraction with GenFind V3

Genomic DNA isolated from various blood collected in heparin, EDTA and citrate tubes was run on the Agilent Genomic DNA ScreenTape to assess quality. (Left) DIN values from all samples isolated with the GenFind V3 kit were all ≥ 9.0, indicating that high-quality and intact gDNAs were recovered. DNA isolated using Supplier A’s kit were of lower quality indicated with a DIN score of 7.8. (Right) Sample traces of the Genomic DNA isolated from tubes containing heparin.

Interested in more data? Explore the GenFind V3 data sheet.

Instructions for Use and Supplemental Protocols*

  GenFind V3 Instructions for Use
  Supplemental Protocol: DNA extraction from up to 2 mL blood
  Supplemental Protocol: DNA extraction from Mouthwash samples
  Supplemental Protocol: DNA Extraction from Bacteria
  Supplemental Protocol: Extraction of DNA from Mucoid Bacteria
  Supplemental Protocol: DNA Extraction from Filamentous Fungi
  Supplemental Protocol: DNA extraction from Zeesan Saliva DNA sample collection kit
  Supplemental Protocol: DNA extraction from fresh/frozen tissue

Specifications

Application Genomic DNA extraction
Technology SPRI paramagnetic bead-based technology
Sample types Whole blood (fresh or frozen), serum, cultured cells, dry blood spots,* mouthwash,* saliva,* Gram-positive and Gram-negative bacteria (E. coli, S. aureus, Salmonella, Corynebacterium, Mycobacterium),* mucoid bacteria (P. aeruginosa),* fungi (A. niger),* lung tissue (fresh or frozen)*
Kit Components and Storage Conditions Lysis bufer LBB (RT), Proteinase K (RT), Binding buffer BBB (4°C), Washing buffer WBB (RT), Washing buffer WBC (RT)
Input Volume Range 50—400 μl
Processing mode Automated or manual
Kit Sizes 50 and 384 preps

How to Automate GenFind V3 gDNA Extraction

When compared to manual operations, the GenFind V3 kit automated on Biomek liquid handlers provides:

  • Reduced hands-on time and increased throughput
  • Option to run the method end-to-end with only setup and tear-down touch points
  • Reduction in pipetting errors
  • Standardized workflow for improved results
  • Quick implementation with demonstrated methods

To learn more about automation of the GenFind V3 gDNA extraction kit on the Biomek i7 Hybrid Genomics Workstation, read our application note.

To discuss the feasibility to automate the GenFind V3 gDNA extraction in your lab with one of our experts complete this form.

GenFind V3 Genomic DNA Extraction Automated on Biomek i-Series Liquid Handler

Resources

Data Sheet

 

Download ↓

Whitepaper

 

Download ↓

Application Note

 

Download ↓

Customer Spotlight

 

Request

Brochure

 

Download ↓

Featured Video

Citations

Sample Type: Archived neonatal blood spots†
Nickels EM et al. (2022). DNA methylation at birth in monozygotic twins discordant for pediatric acute lymphoblastic leukemia. Nature Communications 13, 6077. doi: 10.1038/s41467-022-33677-z.

Sample Type: Hematopoietic progenitor cells (HPCs), Hematopoietic stem cells (HSCs)†
Tran NT et al. (2022). Precise CRISPR-Cas–mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells. Science Advances, 8(22), eabm9106. doi: 10.1126/sciadv.abm9106.

Sample Type: Peripheral blood mononuclear cells (PBMCs)†
Tsuji T et al. (2020). Clonality and antigen-specific responses shape the prognostic effects of tumor-infiltrating T cells in ovarian cancer. Oncotarget, 11(27), 2669–2683. doi: 10.18632/oncotarget.27666.

Sample Type: IMR-90 cells†
Leung C et al. (2022). Chronic stress-driven glucocorticoid receptor activation programs key cell phenotypes and functional epigenomic patterns in human fibroblasts. iScience, 25(9), 104960. doi: 10.1016/j.isci.2022.104960.

技術資料

資料が見つからない場合は、 こちらの検索ページでご確認ください

*Supplemental data from Beckman Coulter Life Sciences
†Customer-published data

Products and demonstrated applications are not intended or validated for use in diagnostic procedures.
Supplemental data and customer-published data references are provided for demonstration only, and are not validated by Beckman Coulter Life Sciences. Beckman Coulter Life Sciences makes no warranties express or implied with respect to this protocol, including warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. Your use of the method is solely at your own risk, without recourse to Beckman Coulter Life Sciences.