培養細胞からのRNA抽出

RNAdvance Cell v2

ろ過や遠心の必要なく、培養細胞からRNAを抽出します。

  • 高品質の核酸が高収量で得られます
  • NGSやPCR解析に使用可能な精製度の高いRNAが抽出可能
  • 手動抽出、自動抽出の両方に対応

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※クイックマニュアル(日本語)はこちらよりご確認ください。

RNAdvance Cell v2

磁性ビーズによる培養細胞からのRNA抽出ワークフロー

Genomics RNAdvance Cell v2 Workflow

機能

培養細胞から96ウェルフォーマットで高品質な完全長RNAを抽出および精製

 

5 x 104 Cellまでの培養細胞からのインタクトなTotal RNA抽出
(5 x 104 Cell 以上の細胞にはAgencourt RNAdvance Tissueをご利用ください)

96ウェルプレートフォーマットでの抽出・精製に最適で、Biomekによる自動化にも対応できます

製品仕様

Application Uses RNA Isolation, RNA Extraction, RNA Sequencing, Nucleic Acid Sample Prep
Format Liquid
Starting Sample Material Cultured Cells
Automated Available Yes
Item Specifications Referenced A47943

Citations

Hore, T. A., von Meyenn, F., Ravichandran, M., Bachman, M., Ficz, G., Oxley, D., . . . Reik, W. (2016, October 25). Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. PNAS, 113(43), 12202-1207. doi:10.1073/pnas.1608679113

  • Used to extract RNA from ESC and EpiSC cell lines

Mesnage, R., Phedonos, A., Biserni, M., Arno, M., Balu, S., Corton, J. C., . . . Antoniou, M. N. (2017, October). Evaluation of estrogen receptor alpha activation by glyphosate-based herbicide constituents. Food and Chemical Toxicology, 108, 30-42. doi:10.1016/j.fct.2017.07.025

  • Used to extract RNA from MCF-7 cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

資料

Title Description
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