OptiMATE Gradient Maker

What is density gradient centrifugation?

Density gradient ultracentrifugation (DGUC) is a centrifuge-based technique for providing superior purification of viral vectors (e.g., isolating full AAV particles from partial and empty capsids), along with other materials (such as plasmid DNA) in gene therapy production workflows. DGUC results in a layered gradient based on the density of materials in the original sample.

What applications is density gradient ultracentrifugation used for?

• Viruses / viral vectors (e.g., AAV)
• Virus-like particles and bacteriophage
• Extracellular vesicles (EVs)
• Proteins / protein complexes
• Lipid nanoparticles
• Synthetic nanoparticles or other nanomaterials
• Nucleic acids / plasmids
• Lipemic separations

What density gradient reagents are compatible with the OptiMATE Gradient maker?

• Cesium Chloride
• Iodixanol (OptiPrep^™)
• Sucrose

What tubes are compatible with the OptiMATE Gradient Maker?

Sizes

• 16 mm (8.9 to 13.5 mL)
• 25 mm (29.9 to 39 mL)
• 38 mm (94 mL)

Formats

• Quick-Seal
• OptiSeal
• Open-Top

Materials

• Polypropylene
• Ultra-Clear

Filling and sealing performance are impacted by tube material, tube size, and tube volume. Not all tubes meeting the above criteria may be suitable. For a complete list of compatible rotors and tubes, please see the instrument brochure and instructions for use (IFU).

Are the tubing lines reusable?

Yes, tubing lines are reusable. They have been tested to three runs.

Can I use 3rd-party tubes with the OptiMATE Gradient Maker?

No, OptiMATE is only compatible with ultracentrifuge tubes provided by Beckman Coulter Life Sciences.