Continuous Cell Culture
Automating Cell-Based Processes
Institutes around the world have hundreds of cell lines frozen in stock that are used in screening activities or tissue engineering. Continuous cell culture provides scientists the ability to readily have assay-ready plates available to test potential agents or to have large quantities of multiple cell lines available for therapies research. Through a continuous cell culture Biomek solution, researchers are able to react quickly to requests from assay-development labs for screening or able to provide their team with batches of cell lines; multiple requests are often received in parallel. The need for automation grows as laboratories increase cell culture throughput in an effort to generate data earlier in their research studies. From seeding, feed, and passaging cells to optimizing media conditions, automation aids in maintaining viable, healthy cells for downstream processes.
Continuous Cell Culture Workflow
Solutions That Meet Your Needs
Minimize the labor-intensive bottlenecks in your lab through automation. As your throughput increases, the Biomek automated continuous cell culture solutions enable you to obtain results you can trace and rely on in an efficient manner, while managing multiple cell lines in parallel to increase the efficiency, and reducing the wait time for bulk quantities of cell lines and assay plates for screening.
Figure 1a, 1b and 1c. Biomek i-Series solutions provide reliable pipetting, integrated data management, and management software for the week to month long processes. In addition, Biomek solutions are developed based on your laboratory’s workflow. From providing a sterile laboratory personal working space to managing the preparation of materials, media system supply solutions, fixed and disposable pipetting requirements, centrifugation, incubation, long-term storage, and integrations that enable monitoring of cell viability and counting, and imaging.
Have an increasing need to produce cell lines and on-hand assay plates more readily? The Biomek i-Series Workstation minimizes the burden of the upfront preparation tasks and cell culture processing.
Biomek Software: Workflow Intelligence at Every Step
Every time your sample moves, data moves. Cell culture workflows are typically performed over the course of several weeks to months at a time. The Biomek Software Suite ensures data integrity to provide peace of mind and schedules activities to streamline automated workflows.
At the heart of every Biomek is the software. Biomek software provides users with the confidence to know their samples are being treated consistently every run, and with every liquid transfer, the data from your samples is being stored. The intuitive, drag and drop user interface makes setting up and maintaining your workflows easy. To meet the evolving needs of cellular workflows, we also offer:
- SAMI EX: provides complete automation and process control by creating planned schedules with the benefits of an optimized, predictable static schedule
- DART (Data Acquisition and Reporting Tool): gathers data and synthesizes runtime information from Biomek log files to capture each manipulation of the sample during the course of the method
- SAMI Process Management Software (SPMS): is a calendar organizational tool that allows the addition, monitoring, and planning of SAMI EX methods and other events as part of user-defined processes
- PowerPack: features advanced tools to make programming data-intensive methods easy
Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions.
Helpful Links
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資料集
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アプリケーションノート
- フローサイトメトリーによる簡単な17 マーカー、 18カラーのヒト血液フェノタイピング
- 21 CFR Part 11 Data Integrity for On-line WFI Instruments
- 8011+ Reporting Standards Feature and Synopsis
- Achieving Compliant Batch Release – Sterile Parenteral Quality Control
- 気中浮遊粒子のモニタリング:ISO21501-4校正のインパクト
- 分析における革命:次世代を担うOptima AUCの概要
- Analyzing Mussel/Mollusk Propagation using the Multisizer 4e Coulter Counter
- Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry
- Automated Cell Plating and Growth Assays
- Automated Cell Transfection and Reporter Gene Assay
- Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi‑CELL XR
- Automated Genomic Sample Prep RNAdvance
- Automated Genomic Sample Prep Sample Quality Control
- Automated salt-assisted liquid-liquid extraction
- Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS
- Automated Transfection Methods
- Automated XTT Assay for Cell Viability Analysis
- Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex
- Automating Biopharma Quality Control to Reduce Costs and Improve Data Integrity
- Automating Bradford Assays
- Automating Cell-Based Processes
- Automating Cell Line Development
- 新機種Avanti J-15遠心機におけるサンプル保護の改善およびサンプル回収率の最大化
- 新機種Avanti J-15遠心機の短時間減速プロファイルによるワークフロー効率の向上
- Avanti JXNによるタンパク質精製ワークフロー
- Avoid the Pitfalls When Automating Cell Viability Counting for Biopharmaceutical Quality Control
- Beer, Evaluation of Final Product and Filtration Efficiency
- Biomek Automated Genomic Sample Prep Accelerates Research
- Biomek Automated NGS Solutions Accelerate Genomic Research
- Biomek i-Series Automated AmpliSeq for Illumina® Library Prep Kit
- Biomek i-Series Automated Beckman Coulter Agencourt RNAdvance Blood Kit
- Biomek i-Series Automated Beckman Coulter Agencourt RNAdvance Cell
- Biomek i-Series Automated Beckman Coulter Agencourt SPRIselect for DNA Size Selection
- Biomek i-Series Automated Beckman Coulter AMPure XP PCR Purification System
- Biomek i-Series Automated IDT® xGen Hybridization Capture of DNA libraries on Biomek i7 Hybrid Genomics Workstation
- Biomek i-Series Automated Illumina Nextera DNA Flex Library Prep Kit
- Biomek i-Series Automated Illumina® Nextera XT DNA Library Prep Kit
- Biomek i-Series Automated Illumina TruSeq DNA PCR-Free Library Prep Kit
- Biomek i-Series Automated Illumina TruSeq® Nano DNA Library Prep Kit
- Biomek i-Series Automated Illumina TruSeq® Stranded mRNA Sample Preparation Kit Protocol
- Biomek i-Series Automated Illumina TruSeq® Stranded Total RNA Sample Preparation Kit Protocol
- Biomek i–Series Automated Illumina® TruSight Tumor 170 32 Sample Method
- Biomek i-Series Automated KAPA HyperPrep and HyperPlus Workflows
- Biomek i-Series Automated New England Biolabs NEBNext® Ultra IITM DNA Library Prep Kit
- Biomek i-Series Automated SurePlex PCR and VeriSeq PGS Library Prep for Illumina®
- Biomek i-Series Automation of the Beckman Coulter GenFind V3 Blood and Serum DNA Isolation Kit
- Biomek i-Series Automation of the Apostle MiniMax™ High Efficiency cfDNA Isolation Kit
- Biomek i-Series Automation of the Beckman Coulter Agencourt DNAdvance Genomic DNA isolation Kit
- 超遠心機および自動分注装置を用いた カーボンナノチューブの調製・精製と 分析用超遠心システムによる解析
- Cell Counting Performance of Vi–Cell BLU Cell Viability Analyzer
- Viability Assessment of Cell Cultures Using the CytoFLEX
- Cell Line Development – Data Handling
- Cell Line Development – Hit Picking
- Cell Line Development – Limiting Dilution
- Cell Line Development – Selection and Enrichment
- Cellular Analysis using the Coulter Principle
- cfDNA Extraction Efficiency Affects NGS Data
- Changes to GMP Force Cleanroom Re-Classifications
- 超遠心分析を用いたバイオ医薬品インスリンのキャラクタリゼーション
- ハンドヘルド型気中パーティクルカウンターで行う クリーンキャビネットの定期的な評価
- Cleanroom Routine Environmental Monitoring – FDA Guidance on 21 CFR Part 11 Data Integrity
- Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System
- Conducting the ISO 14644-3 Cleanroom Recovery Test with the MET ONE 3400
- Considerations of Cell Counting Analysis when using Different Types of Cells
- Consistent Cell Maintenance and Plating through Automation
- Control Standards and Method Recommendations for the LS 13 320 XR
- Counting Efficiency: MET ONE Air Particle Counters and Compliance to ISO-21501
- Critical Particle Size Distribution for Cement using Laser Diffraction
- Use Machine Learning Algorithms to Explore the Potential of Your High Dimensional Flow Cytometry Data Example of a 20–color Panel on CytoFLEX LX
- CytoFLEX
- Detecting and counting bacteria with the CytoFLEX research flow cytometer: II-Characterization of a variety of gram-positive bacteria
- Detecting Moisture in Hydraulic Fluid, Oil and Fuels
- Determination of Size and Concentration of Particles in Oils
- 沈殿および遠心分離を組み合わせたキットを使用しない効率的な核酸分離法
- dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings
- Compensation Setup For High Content DURAClone Reagents
- Echo System-Enhanced SMART-Seq v2 for RNA Sequencing
- Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing and Microbiome Applications
- Efficient Factorial Optimization of Transfection Conditions
- ワクチン開発・製造を推し進める
- Enumeration And Size Distribution Of Yeast Cells In The Brewing Industry
- European Pharmacopoeia EP 2.2.44 and Total Organic Carbon
- Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer
- ベックマン・コールターの遠心機を使用したエクソソーム除去FBSの作製:コスト効果に優れた堅実な選択肢
- Flexible ELISA automation with the Biomek i5 Workstation
- Fully-Automated Cellular Analysis by Flow Cytometry
- Get Control in GMP Environments
- g-Max:ベックマン・コールターの多機能な超遠心用製品群への性能追加
- 遠心機によるナノセルロースの分級
- 超遠心法によりナノ粒子を分級して 正確な粒子径を求める方法
- 超遠心分離法 / 動的光散乱法による 顔料インクの分級と粒子径測定
- HIAC Industrial – Our overview solution for fluid power testing for all applications
- HIAC PODS+ Online Mode & Filter Cart Mode
- HIAC PODS+ versus Parker ACM-20 Performance comparison
- A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System: an application for robust and scalable cancer research and biomarker discovery
- High-Throughput qPCR and RT-qPCR Workflows
- A Highly Consistent BCA Assay on Biomek i-Series
- A Highly Consistent Bradford Assay on Biomek i-Series
- A Highly Consistent Lowry Method on Biomek i-Series
- Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series
- Cell Line Development – Hit Picking
- How to Use Violet Laser Side Scatter Detect Nanoparticle
- フローサイトメーターCytoFLEXを用いた、バイオレットレーザーの側方散乱光を利用してナノ粒子を検出する方法
- HIAC(ハイアック)システムでHRLDセンサーを使用する際の推奨ターボリューム設定
- Automating the Cell Line Development Workflow
- 油中パーティクルカウンターのサンプル調製 繰返し精度を維持するための最善の方法
- ICH Q2 – the Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems
- ICH Q2 – The Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems
- Illumina Nextera Flex for Enrichment on the Biomek i7 Hybrid Genomics Workstation
- Illumina TruSight Oncology 500 Automated on the Biomek NXᴾ Span-8 Genomics Workstation
- Importance of TOC measurement in WFI in light of European Pharmacopoeia change
- Temperature dependence of hydrodynamic radius of an intrinsically disordered protein measured in the Optima AUC analytical ultracentrifuge.
- Isolation of cell-free DNA (cfDNA) from plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation kit— comparison of fully automated, semi-automated and manual workflow processing
- Issues with Testing Jet Fuels for Contamination
- Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity
- Linearity of BSA Using Absorbance & Interference Optics
- 光学式パーティクルカウンターにおける「Long Life Lasers(長寿命レーザー)」の検証
- LS 13 320 XR: Sample Preparation - How to measure success
- Particle Size Analysis Simple, Effective and Precise
- Beckman’s LS 13 320 XR Vs. Malvern Mastersizer
- 20カラー イムノタイピングデータのマシンラーニング アルゴリズムを用いた細胞サブセットの詳細解析
- Flow Cytometric Analysis of auto-fluorescent cells found in the marine demosponge Clathria prolifera
- Matching Cell Counts between Vi–CELL XR and Vi–CELL BLU
- MET ONE 3400
- MET ONE センサーの過酸化水素ガス(VHP)耐性
- 超遠心分離法による 金属コロイド精製と濃縮
- 密度勾配遠心法による 金ナノロッドの分離精製
- Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi CELL XR
- High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 525 Liquid Handler
- Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling
- Miniaturized Enzymatic Assays with Glycerol
- Miniaturized and High-Throughput Metabolic Stability Assay Enabled by the Echo Liquid Handler
- Miniaturized Multi-Piece DNA Assembly Using the Echo 525 Liquid Handler
- Miniaturized Sequencing Workflows for Microbiome and Metagenomic Studies
- Minimal Sample to Sample Carry Over with the HIAC 8011+
- ミネラルウォーター製造工程のばらつき最小化
- Modern Trends in Non‐Viable Particle Monitoring during Aseptic Processing
- Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes
- Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin
- Nanoliter Scale DNA Assembly Utilizing the NEBuilder HiFi Cloning Kit with the Echo 525 Liquid Handler
- ナノ粒子複合材の粒子径測定 - 密度勾配超遠心法と動的光散乱法を用いて -
- Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice
- What to do now that ACFTD is discontinued
- Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids
- Optimizing the Multisizer 4e Coutler Counter for use with Small Apertures
- Optimizing Workflow Efficiency of Cleanroom Routine Environmental Monitoring
- Particle Counting in Mining Applications
- パーティクルカウンター資料集 高圧ガス配管内の清浄度測定- MET ONE 3400ガス校正オプションで ISO 14644の計測を簡単に -
- PCR Reaction Setup and AMPure XP Application
- PCR Reaction Setup Application
- 無菌医薬品製造区域の環境モニタリング
- 無菌医薬品製造のためのペーパーレスによる環境モニタリング
- Analysis of plant genome sizes using flow cytometry: a case study demonstrating dynamic range and measurement linearity
- Principles of Continuous Flow Centrifugation
- Flow Cytometric Approach to Probiotic Cell Counting and Analysis
- Protein purification workflow
- Background Subtraction
- Calibrating the QbD1200 TOC Analyzer
- Detection Limit
- Inorganic Carbon Removal
- JP SDBS Validation
- Method Overview
- Overload Recovery
- QbD1200 Preparing Reagent Solution
- USP System Suitability
- Quality Control Electronic Records for 21 CFR part 11 Compliance
- Using the Coulter Principle to Quantify Particles in an Electrolytic Solution for Copper Acid Plating
- A Rapid Flow Cytometry Data Analysis Workflow Using Machine Learning- Assisted Analysis to Facilitate Identifying Treatment- Induced Changes
- Root Cause Investigations for Pharmaceutical Water Systems
- Scalable Plasmid Purification using CosMCPrep
- Full Automation of the SISCAPA® Workflow using a Biomek NXP Laboratory Automation Workstation
- Specification Comparison of Vi–CELL XR and Vi–CELL BLU
- Specifying Non-Viable Particle Monitoring for Aseptic Processing
- ベックマン・コールターの装置を使用してエクソソームの分離および特性評価を行うための標準化・自動化されたアプローチ
- Streamlined Synthetic Biology with Acoustic Liquid Handling
- Switching from Oil Testing to Water and back using the HIAC 8011+ and HIAC PODS+
- Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube-based DURAClone dry reagent
- k-ファクタを利用したロータの効率比較
- Validation of On-line Total Organic Carbon Analysers for Release Testing Using ICH Q2
- Vaporized Hydrogen Peroxide Decontamination of Vi–CELL BLU Instrument
- Vesicle Flow Cytometry with the CytoFLEX
- Vi CELL BLU FAST Mode Option
- Vi-CELL BLU Regulatory Compliance - 21 CFR Part 11
- A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMax™ on the KingFisher™ Technology
- Automated Research Flow Cytometry Workflow Using DURA Innovations Dry Reagent Technology with the *Biomek i7 Automated Workstation and *CytoFLEX LX Flow Cytometer
- Biomek i7 Hybrid Automated KAPA mRNA HyperPrep Workflow
- Fully Automated Peptide Desalting for Liquid Chromatography–Tandem Mass Spectrometry Analysis Using Beckman Coulter Biomek i7 Hybrid Workstation
- High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 525 Liquid Handler
- Miniaturization of an Epigenetic AlphaLISA Assay with the Echo Liquid Handler and the BMG LABTECH PHERAstar FS
- Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 555 Liquid Handler
- Utilization of the MicroClime Environmental Lid to Reduce Edge Effects in a Cell-based Proliferation Assay
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導入事例 (インタビュー記事)
- 高速冷却遠心機を用いたアデノウイルスベクターの効率的で再現性の良い調製方法
- セルソーターを用いたバイオ燃料高産生藻のハイスループット遺伝子改変オートメーションシステム
- 「細胞のごみ」を分解するオートファジーの 全容に迫る
- B細胞の生成と成熟、維持の研究を創薬にも活かす
- 生殖生物学の基礎研究と現在の生殖補助医療
- 動脈硬化の免疫的な機序から心血管疾患の治療と予後を研究 ― マルチカラーフローサイトメーターで研究を加速 ―
- 10カラーフローサイトメトリーを用いた細胞マーカー解析の有用性
- 研究に最適な卓上遠心機を選択するポイント
- 新しいアプローチでの膠原病疾患治療に向けて
- 機能性抗体で免疫応答を制御し、新しい治療戦略と創薬に結びつける ― 研究を加速するフローサイトメーター ―
- 炎症の観点から疾患の発症メカニズムを解明し、治療薬を創出する
- がん遺伝子パネル解析/クリニカルシーケンスにおけるFFPE組織切片からのゲノムDNA抽出の重要性
- 大学における留学生を対象とした英語による遠心機の安全教育セミナーの有用性
- 大学における共通機器室の機器管理とサポート体制について
- 超遠心法を用いたエクソソームの分離精製
- 免疫研究で漁業の未来を変える - 研究の効率を上げるフローサイトメーター -
- 高精度の迅速診断に向けて - Navios EXによる造血器腫瘍細胞の10カラー解析の導入 -
- iPS 細胞のメカニズムと 安全で効率のよい 樹立・維持法を追究
- X線結晶構造解析のための膜タンパク質精製の基礎
- 密度勾配超遠心法による簡便な細胞小器官の分画
- 粒子間の相互作用研究から、細胞と粒子の相互作用研究へ
- 遺伝子治療ベクターの品質評価における最新事情
- 虹彩細胞からの網膜細胞再生へ ~MoFlo Astriosの役割~
- 堆積地質学の研究における粒度分布測定
- iPS 細胞由来の “ 肝臓の芽 ” の移植で重度肝疾患の治療を目指す
- 患者の自己骨髄細胞を用いて肝硬変を治療する
- 大学の共通機器室での機器管理とサポート体制について
- Fundamentals of Ultracentrifugal Virus Purification
- 総合カタログ
- eBook
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フライヤー
- 10 Things to Consider When Exploring Automation for ELISA
- Cell Line Development
- Cell-Based Assays
- Continuous Cell Culture
- インタビュー記事
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Posters
- AMP 2019: Correlation Between Mutations Found in FFPE Tumor Tissue and Paired cfDNA Samples
- Applications of Ultracentrifugation in Purification and Characterization of Biomolecules
- Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation
- ABRF 2019: Automated Genomic DNA Extraction from Large Volume Whole Blood
- Automated library preparation for the MCI Advantage Cancer Panel at Miami Cancer Institute utilizing the Beckman Coulter Biomek i5 Span-8 NGS Workstation
- Automating Cell Line Development for Biologics
- Cellular Challenges: Taking an Aim at Cancer
- Cell-Line Engineering
- Characterizing the Light-Scatter Sensitivity of the CytoFLEX Flow Cytometer
- ASHG 2019: Comparison between Mutation Profiles of Paired Whole Blood and cfDNA Samples
- ASHG 2019: Correlation Between Mutations Found in FFPE Tumor Tissue and Paired cfDNA Samples
- AACR 2019: Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample
- Mastering Cell Counting
- AACR 2019: Correlation between Mutations Found in FFPE Tumor Tissue and Paired cfDNA Samples
- Preparing a CytoFLEX for Nanoscale Flow Cytometry
- A Prototype CytoFLEX for High-Sensitivity, Multiparametric Nanoparticle Analysis
- AGBT 2019: A Scalable and Automatable Method for the Extraction of cfDNA
- ABRF 2019: Simultaneous DNA and RNA Extraction from Formalin-Fixed Paraffin Embedded (FFPE) Tissue
- A Complete Automation and Reagent Workflow for Analysis of cfDNA: from Plasma to Variants
- 製品の取扱説明書
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ホワイトペーパー
- Centrifugation is a complete workflow solution for protein purification and protein aggregation quantification
- AUC Insights - Analysis of Protein-Protein-Interactions by Analytical Ultracentrifugation
- GMPクリーンルームの清浄度クラスと日常環境モニタリング
- AUC Insights - Assessing the quality of adeno-associated virus gene therapy vectors by sedimentation velocity analysis
- AUC Insights - Sample concentration in the Analytical Ultracentrifuge AUC and the relevance of AUC data for the mass of complexes, aggregation content and association constants
- AccuPlex™SARS-CoV-2標準物質キットを用いたRNAdvance Viral XP RNA抽出キットの特徴付け
- CytoFLEX Platform Flow Cytometers with IR Laser Configurations: Considerations for Red Emitting Dyes
- Hydraulic Particle Counter Sample Preparation
- ベックマン・コールター社製ウイルスRNA抽出溶解緩衝液を用いたCOVID-19疾患ウイルスSARS-CoV-2の不活化
- Liquid Biopsy Cancer Biomarkers – Current Status, Future Directions
- MET ONE 3400+ IT Implementation Guide
- ライフサイエンス分野製品 安全性データシート(SDS/MSDS)
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アプリケーションノート