ゲノム試薬選択ガイド
当社のゲノム試薬製品は、当社Agencourt AMPure XPで使用されている、常磁性ビーズにより核酸を種類とサイズ別に選択的に回収するSPRI（Solid Phase Reversible Immobilization：可逆固定法）テクノロジーを採用しています。当社化学製品はSPRIにより、qPCR、ddPCR、サンガー法シーケンシング、次世代（NGS）シーケンシング、マイクロアレイなどの解析法を支援するハイパフォーマンスな分離・精製・クリーンアッププロトコルを実現しています。
以下のチャートで使用する開始物質を確認し、お客様のマニュアル/自動サンプル調製ワークフローに役立つキットをご覧ください。
| 開始物質 | DNA分離 | RNA分離 | 精製およびクリーンアップ | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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CosMC Prep
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DNAdvance
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FormaPure DNA1
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GenFind v2
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FormaPure
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RNAdvance Blood
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RNAdvance Cell v2
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RNAdvance Tissue
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AMPure XP
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CleanSeq
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RNAClean XP
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SPRIselect
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| 哺乳動物サンプル | |||||||||||||
| 口腔内細胞 |
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| FFPE組織 |
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| FTAスワブ |
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| Oragene唾液採取チューブ |
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| 血漿 |
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| 血清 |
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| 組織 |
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| 全血 |
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| 細胞培養 | |||||||||||||
| 293 T |
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| 付着細胞 |
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| CHO |
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| HeLa |
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| 肝細胞数＜50,000個 |
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| 肝細胞数≧50,000個 |
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| 非付着細胞 |
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| 細菌 | |||||||||||||
| 枯草菌（グラム陽性） |
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| 大腸菌（グラム陰性） |
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| プラスミド | |||||||||||||
| BAC、コスミド、フォスミド |
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| その他の物質 | |||||||||||||
| トリ血液 |
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| 昆虫（ショウジョウバエを含む） |
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| ゼブラフィッシュ |
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| 核酸サンプル | |||||||||||||
| cDNA合成 |
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| ダイターミネーター除去 |
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| In vitro転写 |
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| NGSライブラリ構築手順 |
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| PCR産物 |
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Highlighted Extraction Protocols
Sample Types from Customers and Protocols Developed
| Sample Type | Title of Supplemental Protocol or Kit Suggested |
|---|---|
| PAXgene Blood DNA collection tube | Whole Blood DNA extraction from PAXgene Blood DNA Tube using GenFind V3 |
| FTA/blood spots | DNA extraction from FTA cards or filter paper blood spots using GenFind V3 |
| Large volume blood | DNA extraction from up to 2mL of Whole Blood |
| PBMCs | DNA extraction from a large cell number |
| Archaeological bone samples | Contact PoP Team for help with this sample type |
| Formalin Fixed Autopsy Samples |
Formalin-fixed and non-paraffin-embedded tissue DNA extraction |
| Feces |
Contact PoP Team for help with this sample type |
| Liquid Biopsy |
Apostle MiniMax™ |
| Mouthwash |
DNA extraction from Mouthwash using GenFind V3 |
| Zeesan Saliva |
DNA extraction from Zeesan Saliva DNA sample collection kit using GenFind V3 |
| Swabs |
Isolation of DNA from Buccal Swabs using DNAdvance |
| Urine Pellets |
Contact PoP Team for help with this sample type |
| Fibrotic Tissue |
Contact PoP Team for help with this sample type |
| Fatty Tissue |
Contact PoP Team for help with this sample type |
| Fresh/frozen tissue |
DNAdvance |
| FFPE tissue |
FormaPure XL |
| Fixed Tissue |
Formalin-fixed and non-paraffin-embedded tissue DNA extraction |
| Bacteria (E. coli, Bacillus and others) DNA |
DNA Isolation from Bacteria using GenFind V3 |
| Bacteria (E. coli, Bacillus and others) RNA |
RNA Isolation from Bacteria using RNAdvance Tissue |
| Bacteria (mucoid P. seudomonas) DNA |
Extraction of DNA from Mucoid Bacteria using GenFind V3 |
| Bacteria (mucoid P. seudomonas) RNA |
RNA Isolation from Mucoid Bacteria with RNAdvance Tissue Kit |
| Bacteria in Urine |
Contact PoP Team for help with this sample type |
| Viral RNA or DNA |
Viral Nucleic Acid extraction using RNAdvance Blood |
| Fungi |
DNA extraction from Filamentous Fugni using GenFind V3 |
| Fungus from fingernail clippings |
Contact PoP Team for help with this sample type |
| Yeast |
Contact PoP Team for help with this sample type |
| HEK 293 T Cells, TC cells, C2C12 Cell line |
DNA extraction from cell culture using GenFind V3 |
| High Molecular Weight (HMW) DNA extraction |
Contact PoP Team for help with this sample type |
| DNA and RNA extraction from one sample |
Simultaneous DNA & RNA Extraction from Tissue without Splitting the Lysate |
| Plants (tobacco) |
Contact PoP Team for help with this sample type |
Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.
© 2019 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
Beckman Coulter Life Sciences partners to offer Apostle MiniMax™ products, a trademark of Apostle, a liquid biopsy company.
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