Echo 525リキッドハンドラー

高容量の試薬分注に

生化学・ゲノミクス分野のサンプルおよび試薬分注に適した設計
試薬コストを削減しつつもデータ品質を向上
大容量の試薬分注用リザーバ

新しい液体分注の考え方

Echo 525は、生化学分析用試薬およびゲノミクスサンプルの分注に特化して設計された、初の高速アコースティックリキッドハンドラーです。Echo 525リキッドハンドラーおよびEchoソフトウエア・アプリケーションの導入により、すぐにプロトコルの作成と実行ができるため、煩雑な手順を覚える必要がなく、研究者の皆様はデータの検討に使う時間を増やすことができます。さらに、Echoシステム用に設計されているAccess Laboratory Workstationを使用すれば、Echo 525リキッドハンドラーのユーティリティを拡張でき、さらなる時間節約につながります。

Echo 525 の特長

主な利点

極めて精密かつ思い通りの微量分注により、アッセイのミニチュア化を実現
従来のリキッドハンドラーよりも50～100倍も速くアッセイ系を構築できるため、たった1台でラボの生産性を高めることが可能
Echoソフトウエア・アプリケーションが、多様なアプリケーションにおいて複雑なプロトコルの作成をサポート
アッセイの成功を妨げるピペッティングによるばらつきを排除
Labcyte Access Laboratory Workstationとのインテグレーションにより、スループットを向上させることが可能

複雑なアッセイを迅速に構築

Echo 525リキッドハンドラーの柔軟性、スピード、正確性、および精度は、従来のリキッドハンドラーでは不可能だったアッセイの構築を可能にしました。アコースティック音響エネルギーを採用し、チップやピンツールを用いた従来のリキッドハンドラーが直面していた制約を解消したのです。1度のランで、1枚のソースプレートから様々な液量および粘性粘度の液体を分注し、アッセイ構築を行うことができます。Echoリキッドハンドラーなら、プレート位置が固定されている機器とは異なり、ウェル間で自在に液体を移動させることができるため、実験用プレートを研究者が自らデザインすることが可能で、研究者の皆様を新たな発見へと導きます。

Echo 対応のリザーバで作製した試薬を効率的に分注

Echo 対応のリザーバを用いれば、アッセイ構築や、試薬を多くのウェルに分注する操作を簡単に行うことができます。各マイクロウェルから最大で2.5 mLを分注する能力を備えており、器具を取り変えることなくアッセイを構築できるため、時間とコストを節約できます。

ゲノミクス解析用試薬の柔軟かつ確実な分注

Echo 525リキッドハンドラーは、1回の分注操作で多様な液体を扱うことができます。蒸留水、バッファー、核酸のほか、グリセロールを最大50％まで含有する試薬にも対応しています。Echo 525リキッドハンドラーは、扱える液体種類や分注量の幅広さ、ウェル間での自在な分注機能、およびパワフルなアプリケーションソフトウエアにより、次のような広範なゲノミクスアッセイのワークフローをサポートすることが可能です：

遺伝子合成
PCRおよびqPCRアッセイのセットアップ
RNAおよびDNAの次世代シーケンシングのワークフロー
サンガー法によるシーケンシングのセットアップ
シングルセルサンプルの調製

動画で観るアコースティックテクノロジー

Echoアコースティックドロップエジェクション（ADE）のテクノロジーを動画でご紹介します。ソースプレートから上部に設置されたデスティネーションプレートへ、正確なボリュームの液滴を音波により放出する様子をご覧いただけます。

製品仕様

Drop volume	25 nL
Volume transfer range	25 nL – 5 µL one well to one well
Transfer accuracy	<10% deviation from target volume
Transfer precision	<8% CV
Reagents supported	PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, and antibodies
Source labware compatibility	EchoQualified 384 Polypropylene, 384 Polypropylene Plus, Reservoir, Microplates
Destination labware compatibility	All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-well formats 8 -16 mm in height. Use of 1536-well plates as destination plates is application dependent
Dimensions	53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight	128 kg (283 lbs)
Working envelope	Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical	AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature	21°C ± 5°C (70°F ± 9°F)
Vacuum	House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m³/hr (70.68 cu ft/hr), minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration	No calibration needed

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Title	Description
Echo for Regulated Labs	Complex workflows and stringent success criteria require you to use liquid handling solutions that offer both the flexibility to meet your unique needs and the exceptional reproducibility your work demands
Acoustic Liquid Handling Technology	We have revolutionized liquid handling with an innovative technology that produces faster and more accurate results than traditional pipetting methods. Whether you are involved in drug discovery, compound management, genomics research, synthetic biology, proteomics, functional screening, or other research applications, you will do better science and save money with the Echo acoustic droplet ejection (ADE) technology.
Acoustic liquid handling overview
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation: A Streamlined Workflow to Improve NGS Quality Control Testing	This webinar will discuss a streamlined library preparation method for next-generation sequencing (NGS) that improves quality control testing capability while reducing the total number of steps compared to conventional multiplexed library workflows.
Cell-free and Acoustic Liquid Handling are Helping Tierra Disrupt Natural Product Discovery	If you want to disrupt the natural products market, try combining acoustic liquid handling technology with advances in cell-free approaches. In this webinar, Zachary Sun, CEO of Tierra Biosciences (formerly Synvitrobio), shares how his company is doing just that using the Echo Liquid Handler
Fast Cost-Effective High Throughput Solutions for DNA Assembly	Fast Cost-Effective High Throughput Solutions for DNA Assembly
Automated group testing at scale to enable COVID-19 decision makers - Using Echo for tapestry pooling and high-throughput qPCR setup
Miniaturized Illumina Nextera XT Library Prep using an Integrated Biomek i5 and Echo 525 Liquid Handling Workstation {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	Alex Pierson and Shilpa Parakh from Beckman Coulter Life Sciences present their work on Miniaturized Illumina Nextera XT Library Prep using an Integrated Biomek i5 Multichannel and Echo525 Liquid Handling Workstation at ASHG 2019 in Houston, TX.
Echo Acoustic Liquid Handling Technology {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	Sound waves eject precisely sized droplets from a source onto a microplate, slide, or other surface suspended above the source
5 Years of high-throughput strain engineering with the Echo: lessons from Zymergen Automation presented by Christopher Bremner, Automation Engineer, Zymergen @SLAS2020 {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	Zymergen has used the Echo Liquid Handler to automate strain engineering for many years now. In this talk, Chris reflects on some lessons learned during early Echo usage, provides some tips to maximize throughput of your Echo device, and showcases how Zymergen currently use Echo devices in their workflows.
Off-the-shelf, Echo-based DNA Assembly Automation presented by George McArthur, Arzeda @SLAS2020 {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	In this talk, George describes how his team transformed a series of arduous, complex, time-consuming and error-prone laboratory tasks into an effortless, straightforward, fast and mistake-free workflow using a combination of accessible software and hardware automation.
Biomek i-Series and Echo Acoustic Liquid Handler Integrated Workstation {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	This video highlights the Biomek i-Series and Echo Acoustic Liquid Handler Integrated Workstation
Echo Acoustic Technology	MOVING LIQUIDS with SOUND Delivering Precise, Accurate, and Cost-efficient Liquid Transfers. The Echo Liquid Handler has revolutionized liquid handling with an innovative technology that produces faster and more accurate results than traditional pipetting methods.
Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing and Microbiome Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we detail a method to miniaturize reaction volumes utilized in the Illumina Nextera XT DNA library kit, thereby reducing the overall cost per reaction while maintaining equivalent data to the standard protocol.
Miniaturized Multi-Piece DNA Assembly Using the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In the studies described in this Application Note, the NEB NEBuilder HiFi DNA Assembly Cloning Kit was combined with the Echo 525 Liquid Handler to assemble a construct over five modular pieces.
Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The goal of this experiment was to utilize the Echo 525 Liquid Handler in assembling five, five-piece assemblies by substituting modular DNA pieces at the nanoliter scale.
Tierra Biosciences reveals major molecular discovery {D62418F5-2B8B-4155-AC0E-3ABFBF3E7911}	Today, Tierra Biosciences has multiple cell-free systems representing the diversity of life, spanning gram-positive, gram-negative and eukaryotic species. Using the company’s technology, scientists can easily vary elements of their experiments that are difficult to manage when using cells.
High-throughput next-generation DNA sequencing of SARS-CoV-2 enabled by the Echo 525 Liquid Handler {D62418F5-2B8B-4155-AC0E-3ABFBF3E7911}	Scalability, reproducibility, and precision are characteristics required for high-throughput molecular assays. Using focused sound waves, the Echo 525 Liquid Handler dispenses liquids with 25 nL precision into PCR destination plates. This capability allows laboratories to assemble PCR reactions, on demand, directly from concentrated primer stocks.
Miniaturization of an Epigenetic AlphaLISA Assay with the Echo Liquid Handler and the BMG LABTECH PHERAstar FS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Epigenetics continues to emerge as an important target class for drug discovery and cancer research. As programs scale to evaluate many new targets related to epigenetic expression, new tools and techniques are required to enable efficient and reproducible high-throughput epigenetic screening. The combination of robust and precise instrumentation from Beckman Coulter Life Sciences and BMG LABTECH enable the miniaturization and automation of PerkinElmer AlphaLISA epigenetic assay kits without compromising performance and data quality.
High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Results presented here demonstrate that miniaturized and simplified time-dependent CYP inhibition assays can be efficiently conducted with the Echo Liquid Handler.
Host Cell Residual DNA Testing in Reduced Volume qPCR Reactions Using Acoustic Liquid Handling {611E05FD-FC41-4E23-9498-DA5662F8D4F9}	By integrating the resDNASEQ workflow with Echo Liquid Handlers, the number of serial dilutions were dramatically reduced, the reaction volumes were lowered, and with a dispense resolution of 2.5 nL, an increase in DNA sensitivity was observed. The precise and accurate acoustic transfer ability afforded by the Echo Liquid Handler platform greatly enhances the residual host cell DNA testing workflow.
High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we discuss assay results from miniaturized time-dependent inhibition assays. The time-dependent inhibition for two of the most abundant CYP enzymes, CYP3A4 and 1A2, were evaluated. The results from the Echo Liquid Handler method demonstrate fast, reliable and accurate IC50 values.
Echo System-Enhanced SMART-Seq v4 for RNA Sequencing	Here, we document information and results pertaining to the miniaturization of this process utilizing the Echo 525 Liquid Handler, effectively reducing the reagent cost and processing time for the workflow, addressing current throughput needs of RNA sequencing.
Assay Assembly for Miniaturized Quantitative PCR in a 384-well Format Using the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note highlights the capabilities of the Echo 555 Liquid Handler to dispense into 384-well qPCR plates with total reaction volumes as low as 250 nanoliters. Standard deviations of less than 0.25 and CVs of less than 2% are seen routinely across plates using the low volume dispensing of the Echo Liquid Handler.
Optimized NGS Library Preparation with Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Radiant Genomics and Beckman Coulter Life Sciences have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo 525 Liquid Handler. In addition to cost savings, we demonstrate an increase in sample-processing throughput with the Echo Liquid Handler due to efficient multiplexing, which would otherwise remain impractical using standard liquid handling automation. In addition, the barcoding and combinatorial pooling strategy outlined here can be applied to workflows beyond NGS.
Miniaturized Sequencing Workflows for Microbiome and Metagenomic Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Echo® 525 Liquid Handler can be employed to miniaturize workflows, thus decreasing reagent cost and time spent per sample. Our results show that miniaturizing sequencing workflows is possible and yields high quality data. Here Echo 525 Liquid Handler enables the throughput needed for modern microbiome studies by reducing the time and cost per sample and processing them with speed and accuracy.
Miniaturized 16S rRNA Amplicon Sequencing with the Echo 525 Liquid Handler for Metagenomic and Microbiome Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here, we perform a standard Illumina 16S rRNA amplicon sequencing library preparation at miniaturized scale using the Echo 525 Liquid Handler, effectively reducing reaction volumes and input sample while maintaining sufficient read depth to accurately capture the community. The result is a significantly cost-reduced workflow per sample, saving reagent costs, operational time costs, and valuable sample DNA.
Miniaturized and High-Throughput Metabolic Stability Assay Enabled by the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we discuss how the Echo Liquid Handler provides a platform for conducting a miniaturized high-throughput metabolic stability assay. The Echo Liquid Handler precisely and accurately transfers nanoliter volumes with acoustic energy in a completely contact-free manner. Thus high quality data can be obtained with a small fraction of the volume required by tip-based liquid handling methods.
Automated, high-throughput and low-cost RT-qPCR methods for SARS-CoV-2 surveillance testing using the Echo 525 Liquid Handler	Using this method, the group has been able to study up to 5,000 samples per day with a fast turnaround time of 15 minutes vs. 3 hours for the reaction setup. Moreover, because the Echo is an acoustic and tip-less technology, it reduces usage of disposable tips, which is critically important because of the high demand for tips during the SARS-CoV-2 pandemic.
Echo Liquid Handling for Biochemical Assays	The Echo Liquid Handler helps ensure that samples and reagents are dispensed without risk of contamination or sample degradation — regardless of fluid type.
Echo System-Enhanced SMART-Seq v2 for RNA Sequencing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here, we demonstrate miniaturization of this process on human brain total RNA utilizing the Echo 525 Liquid Handler. We show that this enhanced process can effectively reduce reagent cost for the workflow, and see that genes are not differentially expressed across miniaturization and input variables as shown in Illumina RNAExpress analysis in BaseSpace.
dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The precision and accuracy of the miniaturized reaction volumes for the two kits in an Echo 525 Liquid Handler-enabled workflow was verified by quantifying a library of dsDNA samples in a 384-well polypropylene microplate and cross checking the quantification data against the Qubit dsDNA (HS) Assay Kit.
Streamlined Synthetic Biology with Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Echo Liquid Handler has demonstrated the ability to generate DNA constructs using various assembly chemistries in combination with these modular pieces in a miniaturized workflow.
Nanoliter Scale DNA Assembly Utilizing the NEBuilder HiFi Cloning Kit with the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The goals of these experiments were to utilize the Echo 525 Liquid Handler in assembling both a two-piece and five-piece assembly in miniaturized reaction volumes.
Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: Using the Echo 525 Liquid Handler in combination with the myTXTL Master Mix provides a comprehensive solution for gene expression projects that require rapid high-throughput processing, flexibility, and reproducibility in a low cost assay
Australian Lab Achieves High-Throughput Genotyping by Amplicon Sequencing Using Echo 525 & Access Systems {D62418F5-2B8B-4155-AC0E-3ABFBF3E7911}	<p style="margin-bottom: 12pt;"><span style="color: black; background: white; letter-spacing: 0.1pt;">The BRF lab typically analyzes 65,000+ mouse samples/year, which translates to 110,000+ genotypes annually, or an average of 2,000 genotypes each week. </span></p> <p style="margin-bottom: 12pt;"><span style="color: black; background: white; letter-spacing: 0.1pt;">They are currently developing a new highly multiplex amplicon protocol, both reducing costs and with the ability to ramp up to <em>double</em> their current throughput—to 4,000 samples/run <a></a><a><span>each week</span></a></span><span style="color: black; background: white; letter-spacing: 0.1pt;">—a goal made feasible using an Echo 525 acoustic liquid handler.</span></p> <div> </div>
Whole Genome Sequencing of Microbial Communities for Scaling Microbiome and Metagenomic Studies Using the Echo 525 Liquid Handler and CosmosID {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this study, we demonstrate that the Echo 525 Liquid Handler can be used to miniaturize WGS of microbial communities. Raw data can be processed, analyzed, and ready for interpretation within the hour, by utilizing CosmosID’s best-in-class microbiome bioinformatics platform.
Echo System-Enhanced SMART-Seq v2 for RNA Sequencing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: We demonstrate miniaturization of this process on human brain total RNA utilizing the Echo 525 Liquid Handler.
Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing and Microbiome Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we detail a method to miniaturize reaction volumes utilized in the Illumina Nextera XT DNA library kit, thereby reducing the overall cost per reaction while maintaining equivalent data to the standard protocol.
Streamlined Synthetic Biology with Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Echo Liquid Handler has demonstrated the ability to generate DNA constructs using various assembly chemistries in combination with these modular pieces in a miniaturized workflow.
Miniaturized Multi-Piece DNA Assembly Using the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In the studies described in this Application Note, the NEB NEBuilder HiFi DNA Assembly Cloning Kit was combined with the Echo 525 Liquid Handler to assemble a construct over five modular pieces
Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: Using the Echo 525 Liquid Handler in combination with the myTXTL Master Mix provides a comprehensive solution for gene expression projects that require rapid high-throughput processing, flexibility, and reproducibility in a low cost assay
Long-Term Compound Storage in Acoustically Enabled Tubes: Theory and Experiment {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Acoustic transfer of compounds between microplates by Echo liquid handling instruments has facilitated high-throughput drug discovery at a reduced cost.
Miniaturization of a Five Piece PIK3CA Plasmid Assembly by Utilizing NEBuilder® HiFi DNA Assembly Cloning Kit and Echo 525 Liquid Handler {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	The NEBuilder HiFi DNA Assembly Cloning Kit is one chemistry that, in combination with interchangeable DNA fragments, enables rapid construct generation.
Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	In vitro transcription (TX)-translation (TL) is a rapidly developing technology with vast potential for synthetic biology and bio-engineering.
Reduced Animal Usage and Decreased Study Cost through Microsampling Approaches During Preclinical Safety Testing {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Serial microsampling of animals reduces the overall number of animals sampled and euthanized for toxicology studies
Efficient Plasmid Validation By Multiplexing and Miniaturizing Illumina Nextera XT with the Echo 525 Acoustic Liquid Handler {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Synthetic biology is an interdisciplinary science with the potential to impact several academic and industrial applications including the creation of novel therapeutics and vaccines, plant science and biofuels, as well as bio-based chemical manufacturing capabilities that involves the application of engineering principles to biology.
dsDNA Quantitation with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Poster: Precise dsDNA quantitation is a requirement for many workflows in Genomic research. A variety of quantitation kits are commercially available for diagnostics, synthetic biology, and next-generation sequencing applications.
マルチプレックス全ゲノムシーケンシングおよびマイクロバイオームアプリケーションのためのIllumina Nextera XTライブラリ調製の効果的な微量化 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	研究コストと時間削減への貢献する、リアクションボリュームの微量化メソッドについて解説します。
High-throughput qPCR and RT-qPCR Workflows Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB® Luna® Reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: We show that NEB Luna qPCR reagents offer strong performance in high-throughput qPCR applications and are readily compatible with automated reaction setup, here using the Beckman Echo 525 acoustic liquid handler.
Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: The Echo Liquid Handler enables lower-cost methods and workflows to produce high-quality synthetic DNA constructs which expands design-based testing with higher throughput and affords the scientist a broader biological landscape to interrogate.
Product Solutions for Viral Research Workflows {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Brochure: Discover product solutions for sample collection, isolation and preparation, virus identification, antibody analysis, and vaccine and therapy development.
Echo Series Liquid Handlers {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Unlike traditional liquid handlers, the Echo system uses sound energy to precisely transfer liquid without contact or using pipette tips, nozzles or tubing. Designed for diverse applications in scientific research, the Echo Liquid Handler combines the innovative technologies of Acoustic Droplet Ejection and Dynamic Fluid Analysis to accurately and reliably transfer a wide range of fluids. Across many scientific disciplines including drug discovery, genomics, synthetic biology, and functional studies,
Echo Acoustic Liquid Handling for Synthetic Biology {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Advantages of the Echo Liquid Handler in Synthetic Biology Compared to Tip-based Liquid Handlers
Echo System-Enhanced SMART-Seq v4 for RNA Sequencing	Technical Note: We document information and results pertaining to the miniaturization of this process utilizing the Echo 525 Liquid Handler, effectively reducing the reagent cost and processing time for the workflow, addressing current throughput needs of RNA sequencing.
Nanoliter Scale DNA Assembly Utilizing the NEBuilder HiFi Cloning Kit with the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Technical Note: The integration of a Echo 525 Liquid Handler into a DNA construction pipeline, such as one using the NEB NEBuilder HiFi kit, allows for significant volume reductions as well as workflow enhancements with both complex and simple plasmid designs.
Miniaturized Sequencing Workflows for Microbiome and Metagenomic Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here, we introduce experiments of various sequencing workflows, miniaturizing them at least 10-fold. Our results show that miniaturizing sequencing workflows is possible and yields high quality data
Optimized NGS Library Preparation with Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Radiant Genomics and Beckman Coulter Life Sciences have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo 525 Liquid Handler.
Echo System-Enhanced SMART-Seq v2 for RNA Sequencing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As next-generation sequencing continues to integrate and expand into core research functions, researchers are enabled to ask more questions about the transcriptome, with greater degrees of complexity.
dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Precise dsDNA quantification is a requirement for many genomics workflows including synthetic biology, and next-generation sequencing.

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